Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters.
Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-7, Mol.
Individual members of this family have identical predicted secondary structures with 12 transmembrane domains.
Both N and C-termini are predicted to be cytoplasmic.
Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 using chemiluminescent detection
ELISA: 1:10,000-1:50,000.
G3900-15F Control peptide can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.05% sodium azide.
Specificity：Recognizes rat Glut-2.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：A 16 amino acid peptide sequence corresponding to the exoplasmic loop between helices 1 and 2 of rat liver Glucose Transporter. Conjugated to KLH. Species homology sequence: 93% mouse, human and 84% in ovine.
