Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters.
Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-13, Mol.
Wt.
40-80kD) and sodium glucose co-transporter- 1 (SGLT-1, 662aa; ~75kD).
Individual member of this family have identical predicted secondary structures with 12 transmembrane domains.
Both N and C-termini are predicted to be cytoplasmic.
Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue- specific targeting.
Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics.
Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-10ug/ml using ECL.
Identifies a major band at about 42-45kD from purified rat brain membranes
ELISA: 0.5-1ug/ml.
Control peptide can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Recommended Control Peptide:G3900-05L: GLUT 1 (Glucose Transporter 1) Control Peptide
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes human GLUT 1 (Glucose Transporter 1). Antibodies made to this sequence from this region have detected Glut-1 from human (RBC) and rabbit brain and mouse 3T3 L1 fibroblasts and rat brain adipocytes. It also recognizes the Hep G2 type transporter and Glut-1 from Caco-2 cells (3).
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：A synthetic peptide correesponding to 15aa near the C-terminus, cytoplasmic domain of human Glut-1 (KLH). Species Sequence Homology: porcine, bovine, rabbit, mouse and rat; 100%.
