Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters.
Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins ( Glut-1 to Glut-7 , MW 40-60kD) and Sodium Glucose Co-Transporter-1 (SGLT-1, 662aa; ~75kD).
Individual member of this family have identical predicted secondary structures with 12 transmembrane domains.
Both N- and C-termini are predicted to be cytoplasmic.
Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting.
Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics.
Glut-1 mediates glucose transport into red cells, throughout the blood brain barrier and supply glucose to most cells.
Glut-2 provides glucose to the liver and pancreatic cells.
Glut-3 is the main transporter in neurons.
Glut-4 is primarily expressed in muscle and adipose tissue and regulated by insulin.
Glut-5 transports fructose in intestine and testis.
Glut-6 is a pseudogene and unlikely to be expressed at the protein level.
Glut-7 is expressed in liver and other gluconeogenic tissues and mediates glucose flux across endoplasmic reticulum membrane.
Glut-8 is found in adult testis and placenta.
Human Glut-9 is expressed in spleen, peripheral leukocytes and brain.
Human Glut-10 (541aa, chromosome 20q13.1; ~30-35% homology with Glut-3 and Glut-8) has been identified as a candidate gene for NIDDM susceptibility.
It is widely expressed with highest levels in liver and pancreas.
Glut-11 (496aa, chromosome 22q11.2; ~41% identity with Glut-5) is expressed in heart and skeletal muscle.
Glut-12 (human 617aa, , monkey 621aa; ~ 50kD; ~30% homology with Glut-4 and 40% with Glut-10) is expressed in skeletal muscle, adipose tissue, and small intestine.
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000.
Identifies a major band at about 42-45kDELISA: 1:10,000-50,000.
Control Peptide (G3900-05E) can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Control Peptide:ELISA: G3900-05E, GLUT 1, Control Peptide, Human
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes GLUT 1. Does recognize Hep G2 type transporter and Glut-1 from Caco-2 cells. Species Crossreactivity: Human RBC, rabbit brain, mouse 3T3 L1 fibroblasts, rat brain adipocytes. Species Sequence Homology: Porcine, bovine, rabbit, mouse, rat: 100%; Chicken: 75%.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Hu Po Rb Rt
Immunogen：Synthetic peptide 13aa sequence near the C-terminus of human Glut-1 (KLH coupled).
