Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters (Glut or SLC2A family).
Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 9 proteins (Glut-1 to Glut-14, MW 40-60kD).
Individual members of this family have identical predicted secondary structures with 12 transmembrane domains.
Both N and C-termini are predicted to be cytoplasmic.
There is a large extracellualr domain between TM1-TM2 and a cytoplasmic domain between TM6-TM7.
Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting.
Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics.
Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier.
It is ubiquitously expressed and transport glucose in most cells.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Detects a major band ~42-45kD using Glut-1 positive control from purified rat brain membranes (1).
Not recommended to heat the samples prior to loading to prevent Glut protein aggregation
ELISA: 0.5-1ug/ml.
Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Control Peptide: G3900-01D
Storage and Stability:For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as liquid in PBS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes mouse Glut-1. No significant sequence homology with other Glut proteins. Species sequence homology: human, rat, rabbit, bovine and porcine (100%); chicken (90%).
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide consisting of 12aa sequence near the C-terminus of mouse/rat brain glucose transporter-1 (Glut-1) (KLH coupled).
