GH secretagogues (GHSs) are synthetic compounds that are potent stimulators of GH release.
GHSs, act through a novel orphan G-protein-coupled receptor (GPCR), the GHS receptor (GHS-R).
Ghrelin has been purified and identified from rat and human stomach as the endogenous ligand for the GHS-R.
The rat and human mature Ghrelin (28-aa) are produced from 117aa precursor.
In rat stomach, a 28aa Ghrelin and des-Gln14-Ghrelin (deletion of Gln14) are produced due to alternative splicing of Ghrelin mRNA.
The activity of both Ghrelins is the same.
Ghrelin has an unusual modification at Ser3 residue that is n-octanoylated and it is essential for biological activity.
Rat Ghrelin is expressed in the stomach, small and large intestines, and brain regions (hypothalamic arcuate nucleus) that are involved the regulation of food intake.
Both Ghrelin and GHS-R expression is detected in the heart, suggesting that Ghrelin might have some cardiovascular effects.
GHS-R (rat 364aa; human 366aa, ~90% sequence homology) is a receptor for GHRP and non-peptide, low mol wt.
secreatgogues.
This receptor is coupled to G-a11.
Two isoforms of GHS-R have been reported: isoform 1a (human 36aa) and type 1b (289aa and only 5 TM domains) appears not to bind the ghrelin.
GHS-R displays the typical structure of GPCR with TM domains, extracellular N-terminus and intracellular C-terminus.
It is expressed in not only in the pituitary and hypothalamus but also in the hippocampus, pancreas, and neuroendocrine tumors, including somatotropinomas and rat GH3 cells.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot:1-10ug/ml using ECL.
ELISA: 1:10,000-1:100,000 using 50-100ng G2033-75N control peptide/well
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Specificity：Recognizes rat ghrelin receptor at ~27kD in rat brain. No crossreactivity with type 1b GHS-R. No significant sequence homology exists with other GPCRs.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide corresponding to 20aa within the cytoplasmic C-terminus of rat Grehlin Receptor (KLH). Species sequence homology: mouse: (100%); human, sheep, porcine: (95%).
