GH secretagogues (GHSs) are synthetic compounds that are potent stimulators of GH release.
GHSs, act through a novel orphan G-protein-coupled receptor (GPCR), the GHS receptor (GHS-R).
Ghrelin has been purified and identified from rat and human stomach as the endogenous ligand for the GHS-R.
The rat and human mature Ghrelin (28-aa) are produced from 117 amino acids precursor.
In rat stomach, a 28-aa Ghrelin and des-Gln14-Ghrelin (deletion of Gln14) are produced due to alternative splicing of Ghrelin mRNA.
The activity of both Ghrelins is the same.
Ghrelin has an unusual modification at Ser3 residue that is n-octanoylated and it is essential for biological activity.
Rat Ghrelin is expressed in the stomach, small and large intestines, and brain regions (hypothalamic arcuate nucleus) that are involved the regulation of food intake.
Both Ghrelin and GHS-R expression is detected in the heart, suggesting that Ghrelin might have some cardiovascular effects.
GHS-R (rat 364 aa; human 366 aa, ~90% sequence homology) is a receptor for GHRP and non-peptide, low mol wt.
secreatgogues.
This receptor is coupled to G-a11.
Two isoforms of GHS-R have been reported: isoform 1a (human 366 aa) and type 1b (289 aa and only 5 TM domains) appears not to bind the ghrelin.
GHS-R displays the typical structure of GPCR with TM domains, extracellular N-terminus and intracellular C-terminus.
It is expressed in not only in the pituitary and hypothalamus but also in the hippocampus, pancreas, and neuroendocrine tumors, including somatotropinomas and rat GH3 cells.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 using ECL.
ELISA: 1:10,000-1:100,000 using 50-100ng control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide: G2033-75N
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes rat Ghrelin Receptor. Species sequence homology: mouse (100%), human, sheep, and porcine (95%).
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：A 20 aa peptide sequence within the cytoplasmic C-terminus of Rat GHS-R (KLH.)
