Growth hormone (GH), produced and released from the anterior pituitary, controls body growth, carbohydrate-protein-lipid metabolism and water-electrolyte balance.
GH secretagogues (GHSs) are synthetic compounds that are potent stimulators of GH release.
GHSs act through a novel orphan G-protein-coupled receptor (GPCR), the GHS receptor (GHS-R).
Ghrelin ('ghre' is the Proto-Indo-European root of the word "grow") has been purified and identified from rat and human stomach as the endogenous ligand for the GHS-R.
The rat and human mature Ghrelin (28-aa) are produced from 117aa precursor.
In rat stomach, a 28aa Ghrelin and des-Gln14-Ghrelin (deletion of Gln14) are produced due to alternative splicing of Ghrelin mRNA.
The activity of both Ghrelins is the same.
However, des-Gln14-Ghrelin is only present in low amounts in the stomach, indicating that Ghrelin is the major active form.
Ghrelin has an unusual modification at Ser3 residue that is n-octanoylated and it is essential for biological activity.
Ghrelin is the first known example of a bioactive peptide modified by an acyl acid.
Applications:Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-10ug/ml using ECL.
Full length ghrelin is ~3.5kD
ELISA: 1:10,000-1:100,000 using 50-100ng control peptide/well
Immunohistochemistry: 10-30ug/ml in formaldehyde fixed, paraffin embedded tissues
Optimal dilutions to be determined by the researcher.
Control Peptide:G2033-75F: Ghrelin, Human Control Peptide
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.1 BSA.
Specificity：Recognizes human ghrelin.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 13aa (n-octanoylated at Ser3) from the N-terminus of mature human ghrelin, conjugated to KLH. Species Sequence Homology: porcine: (94%); bovine, canine, mouse and rat: (84%). Des-ghrelins have the same sequence.
