84-1043-82　Ghrelin 100ul　G2033-75D

特徴

• Growth hormone (GH), produced and released from the anterior pituitary, controls body growth, carbohydrate-protein-lipid metabolism and water-electrolyte balance.
• GH secretagogues (GHSs) are synthetic compounds that are potent stimulators of GH release.
• GHSs, act through a novel orphan G-protein-coupled receptor (GPCR), the GHS receptor (GHS-R).
• Ghrelin ('ghre' is the Proto-Indo-European root of the word "grow") has been purified and identified from rat and human stomach as the endogenous ligand for the GHS-R.
• The rat and human mature Ghrelin (28-aa) are produced from 117 amino acids precursor.
• In rat stomach, a 28-aa Ghrelin and des-Gln14-Ghrelin (deletion of Gln14) are produced due to alternative splicing of Ghrelin mRNA.
• The activity of both Ghrelins is the same.
• However, des-Gln14-Ghrelin is only present in low amounts in the stomach, indicating that Ghrelin is the major active form.
• Ghrelin has an unusual modification at Ser3 residue that is n-octanoylated and it is essential for biological activity.
• Ghrelin is the first known example of a bioactive peptide modified by an acyl acid.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000-1:5000 (ECL) ELISA: 1:10,000-1:100,000.
• Using 50-100ng control peptide/well
• Optimal dilutions to be determined by the researcher.
• Recommended Control Peptide:G2033-75F: Ghrelin, Human Control Peptide
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.