Galanin is a 29 aa C-terminally amidated (30 aa, non-amidated in humans), highly conserved but unique neuroendocrine peptide originally isolated from intestine.
The first 14 aa are fully conserved in almost all species.
Galanin is found in the brain and the gut.
It modulates a variety of physiological processed including cognition/memory, sensory/pain processing, neurotransmitter/hormone secretion, and feeding behavior.
Several N-terminally elongated (-7-29 and -9-29) or truncated biologically active forms of galanin have also been isolated.
Galanin antagonists are chimeric peptides generated by linking the amino terminal portion of galanin to substance P (galantide, M15), bradykinin (M35), the neurokinin antagonist spantide (C7) or an idealized alpha helical region (M40) (see review in refs 2 by Kask et al 1995).
Galanin mediated its biological effects by interacting with high affinity cell surface G-protein coupled receptors (GALR1-3).
Rat GALR3 (human 368 aa) encodes a protein of 370 aa with 35% and 52% identity with GALR1 and GALR2.
The rat and human GALR3 are ~90% conserved.
Rat GALR3 is expressed in heart, spleen, and testes.
Galanin binding to GALR3 can be displaced by galanin and galanin analogues.
However, human galanin, galanin (1-16), and M40 show lower affinity to GALR3.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:100,000 using 50-100ng control peptide per wellWestern Blot: 1:1000-1:10,000
Optimal dilutions to be determined by the researcher.
Note: The appropriate control immunogenic peptide (G1043-15H) is available to confirm specificity of antibodies.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, containing 40% glycerol and 0.05% sodium azide.
Specificity：Mouse peptide sequence has 100% homology with rat (20/20 aa; 100%) and human (16/20 aa; 80%) GALR3. No significant homology is seen with GLAR1, GALR2 or any other G-protein coupled receptors. Antibody crossreactivity with GALR3 from various species is not known.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：A 20 aa peptide sequence within the cytoplasmic, C-terminus of mouse GLAR3 (1) was coupled to KLH.
