84-1043-75　Galanin 100ug　G1043-01A

特徴

• Galanin is a 29aa C-terminally amidated (30 aa, non-amidated in humans), highly conserved but unique neuroendocrine peptide originally isolated from intestine.
• The first 14aa are fully conserved in almost all species.
• Galanin is found in the brain and the gut.
• It modulates a variety of physiological processed including cognition/memory, sensory/pain processing, neurotransmitter/hormone secretion, and feeding behavior.
• Several N-terminally elongated (-7-29 and -9-29) or truncated biologically active forms of galanin have also been isolated.
• Galanin antagonists are chimeric peptides generated by linking the amino terminal portion of galanin to substance P (galantide, M15), bradykinin (M35), the neurokinin antagonist spantide (C7) or an idealized alpha helical region (M40) Galanin mediated its biological effects by interacting with high affinity cell surface receptors.
• Pharmacological studies with several peptide antagonists and antagonists of galanin receptor suggest the existence of multiple receptor subtypes, and three galanin receptors (GAL-R1, GAL-R2, and GAL-R3) have been clone thus far.
• These receptors belong to the family of G-protein coupled receptor superfamily, characterized by seven transmembrane domains.
• Applications:Suitable for use in Western Blot, ELISA and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA:1:10,000-1:100,000, using 50-100ng of G1043-01D control peptide/wellWestern Blot: 1-10ug/ml (ECL)Immunohistochemistry: 10-30ug/ml in formaldehyde fixed, paraffin-embedded tissues
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.