GABA (g-amino butyric acid) is the most abundant neurotransmitter in mammalian brain.
GABA exerts its effects through ionotropic ligand-gated GABAA, GABAC and GABAB receptors (GABABRs).
A family of GABA-A receptors subtypes exists, which are generated by alternative splicing of alpha 1-6, beta 1-4, gamma 1-4, delta, epsilon, pie, theta, and rho1-3 to form a heteromeric (pentameric) protein complexes.
Various GABA-A subunits show distinct patterns of temporal and spatial expression that may imply its tissue specific physiological role (1).
GABA A (GAA) receptor proteins (450-627 aa) are characterized by the presence of a cleavable signal peptide, a large extracellular N-terminus, 3 TM (transmembrane) domains, a large cytoplasmic domain followed by TM4 and C-terminal extracellular domain.
The regions between TM3-4 and the large cytoplasmic loop are least conserved among various GAA subunits, which may confer subunit specific functionality.
GAA genes are distributed as clusters throughout the human genome (chromosomes 4, 5, 15, and X; delta subunit on chromosome 1).
GAA in the brain are the targets of many clinically important drugs.
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blotting(1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL.
ELISAControl peptide can be used to coat ELISA plates at 1ug/ml and detected with antibodies (1:10-50K for neat serum and 0.5-1ug/ml for affinity pure).
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：supplied as liquid in PBS, pH 7.4 and 0.1% BSA, 0.02% sodium merthiolate, 40% glycerol.
Specificity：The 12 AA rat control peptide is 100% conserved in human and chicken (2). No significant homology is detected with other GAA subtypes or other receptors. The control peptide, because of its small size (<3kD) is not recommended for Western. It should be used in ELISA or antibody blocking studies to confirm specificity of antibodies
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ch Hu Rt
Immunogen：A 12 AA Peptide sequence near the C-terminus of rat GAB2 subunit (2). The peptide was coupled to KLH
