GABA is a major inhibitory neurotransmitter and the GABAergic transmission is terminated by the rapid Na+/Cl-dependent uptake of through GABA transporters.
It has been subdivided into neural and glial uptake systems on the basis of pharmacological properties.
Recently, molecular cloning studies have identified multiple subtypes of GABA transporters (GAT1, GAT2, GAT3; and betaine GABA transporter (BGT-1).
There is ~50% homology between various GABA transporter subtypes.
GABA transporters are predicted to contain 12 potential transmembrane domains.
The NH2 and COOH-termini are predicted to be intracellular.
Two of the high affinity (Km~10 uM) rat GABA transporters (GAT2 and GAT3/GAT-B) share higher amino acid identity (68% and 65%, respectively) with the kidney betaine transporter than with GAT-1 (52% AA identity).
GAT1 and GAT3 have been detected in various parts of the brain while GAT2 is found in many tissues.
It appears that GAT1 and GAT3 are involved in distinct GABAergic transmission while GAT2 may be important in non-neural functions.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using Chemiluminescence technique.
We recommend the use of 0.5-1% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands
ELISA: 0.5-1ug/ml.
Control peptide can be used to coat plates at 1ug/ml.
Immunohistochemistry: 2-10ug/ml in paraformaldehyde fixed sections of tissues.
Optimal dilutions to be determined by the researcher.
Control Peptide:G1015-78 GABA Transporter 3, Rat Control Peptide
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as liquid in PBS, pH 7.4 and 0.1% BSA, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes rat GAT3 without significant homology to any other known eukaryotic protein. Species sequence homology: Mouse-100%, human-92%.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Rt
Immunogen：A 15 AA synthetic peptide near the carboxy terminus of rat GAT3 (3). The peptide was synthesized with an added cysteine amino terminal (KLH).
