84-1043-70　GABA Transporter 3 (GAT-3 (Gamma aminobutyrIc acid) 100ul　G1015-76

特徴

• GABA is a major inhibitory neurotransmitter and the GABAergic transmission is terminated by the rapid Na+/Cl-dependent uptake of through GABA transporters.
• It has been subdivided into neural and glial uptake systems on the basis of pharmacological properties.
• Recently, molecular cloning studies have identified multiple subtypes ofGABA transporters (GAT1, GAT2, GAT3; and betaineGABA transporter (BGT-1).
• There is ~50% homology between various GABA transporter subtypes.
• GABA transporters are predicted to contain 12 potential transmembrane domains.
• The NH2 and COOH- termini are predicted to be intracellular.
• Two of the high affinity (Km~10 uM) rat GABA transporters (GAT2 and GAT3/GAT-B) share higher amino acid identity (68% and 65%, respectively) with the kidney betaine transporter than with GAT-1 (52% AA identity).
• GAT1 and GAT3 have been detected in various parts of the brain while GAT2 is found in many tissues.
• It appears that GAT1 and GAT3 are involved in distinct GABAergic transmission while GAT2 may be important in non-neural functions.
• Applications:Suitable for use in ELISA, Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000.
• Recommend the use of 0.5-1% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.