84-1043-68　［取扱停止］GABA Transporter 2 (GAT-2 (Gamma aminobutyrIc acid) 100ul　G1015-71

特徴

• GABA is a major inhibitory neurotransmitter and the GABAergictransmission is terminated by the rapid Na+/Cl-dependent uptake of through GABA transporters.
• It has been subdivided into neural and glial uptake systems on the basis of pharmacological properties.
• Recently, molecular cloning studies have identified multiple subtypes of GABA transporters (GAT1, GAT2, GAT3; and betaine GABA transporter (BGT-1).
• There is ~50% homology between various GABA transporter subtypes.
• GABA transporters are predicted to contain 12 potential transmembrane domains.
• The NH2 and COOHterminiare predicted to be intracellular.
• Two of the high affinity(Km~10 uM) rat GABA transporters (GAT2 and GAT3/GAT-B) share higher amino acid identity (68% and 65%, respectively) with the kidney betaine transporter than with GAT-1 (52% AA identity).
• GAT1and GAT3 have been detected in various parts of the brain while GAT2 is found in many tissues.
• It appears that GAT1 and GAT3 are involved in distinct GABAergic transmission while GAT2 may be important in non-neural functions.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000.
• ELISA: 1:10,000-1:50,000.
• Control peptide can be used to coat ELISA plates at 1ug/ml and detected with antibodies
• Optimal dilutions to be determined by the researcher.
• Control Peptide:G1015-72: GABA Transporter 2, Rat Control Peptide (GAT-2, Rat (Gamma aminobutyrIc acid)
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.