Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using Chemiluminescence technique We recommend the use of 0.5-1% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands
ELISA: 0.5-1ug/ml for affinity purified.
Control peptide can be used to coat ELISA plates at 1ug/ml
Immunohistochemistry/Immunofluorescence: 2-10ug/ml in paraformaldehyde fixed sections of tissues.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in100mM Tris, pH 7.5, 0.2% BSA, 40% glycerol
Specificity：Recognizes rat GAT1. Species Sequence Homology: mouse and human: 100%. No significant homology with any other known eukaryotic protein.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Rt
Immunogen：A 19 AA synthetic peptide in the middle of rat GAT1. The peptide was synthesized with an added cysteine amino terminal and conjugated to KLH.
