GABA (g-amino butyric acid) is the most abundant neurotransmitter in mammalian brain.
GABA exerts its effects through ionotropic ligand-gated GABAA, GABAC and GABAB receptors (GABABRs).
Presynaptic GABABRs inhibit neurotransmitter release by down regulating high voltage activated Ca+2 channels, whereas postsynaptic GABABRs decrease neuronal excitability by activating a prominent inwardly rectifying K+ (Kir) conductance that underlies the late inhibitory postsynaptic potential.
Two N-terminal splice variants GABABR1 (also called GBR1), GABABR1a (GBR1a, rat 960aa, apparent mol wt ~130kD; human 961aa) and GABABR1b (GBR1b; rat 844aa, apparent MW 100kD; human 844aa) have been cloned and characterized.
GABABRs are characterized by the presence of a cleavable signal peptide, a large extracellular N-terminus, 7 TM domains, and C-terminal, cytoplasmic domain.
GABABRs are expressed in all major areas of the brain.
Recently GABABR2 (or GBR2) has been cloned from rat (940aa) and human (941aa).
GBR2 forms heterodimer with GBR1 through interaction at the intracellular C-terminal regions.
Heterodimerization appears to be important in transporting GBR1 to the membrane and expression of functional receptors.
GBR2 also colocalized with GBR1.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Control peptide can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Control Peptide:G1015-44D: GABA Receptor B 1b, Rat, Control Peptide (GBR1b)
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for 12 months after receipt at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as liquid in PBS, 0.1% BSA, 0.05% sodium azide
Specificity：Recognizes rat GBR1b. Peptide sequence is 100% conserved in various alternatively spliced rat GBR1b (844aa), GBR1c (875aa) and GBR1d (812aa). No significant sequence homology is detected with GBR1a or GBR2 or other GABA receptors.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo Rt
Immunogen：Synthetic peptide crresponding to a 16aa sequence near the N-terminus, extracellular domain of rat GBR1b, conjugated to KLH. Species Sequence Homology: mouse: 100%; human: 93%.
