GABA (g-amino butyric acid) is the most abundant neurotransmitter in mammalian brain.
GABA exerts its effects through ionotropic ligand-gated GABAA, GABAC and GABAB receptors (GABABRs).
Presynaptic GABABRs inhibit neurotransmitter release by down regulating high voltage activated, Ca+2 channels, whereas postsynaptic GABABRs decrease neuronal excitability by activating a prominent inwardly rectifying K+ (Kir) conductance that underlies the late inhibitory postsynaptic potential.
Two N-terminal splice variants GABABR1 (also called GBR1), GABABR1a (GBR1a, rat 960 aa, apparent mol wt ~130kD; human 961 aa) and GABABR1b (GBR1b; rat 844 aa, apparent MW 100kD; human 844) have been cloned and characterized.
Human and rat GBR1a and GBR1b share 99% sequence homology.
GABABRs are characterized by the presence of a cleavable signal peptide, a large extracellular N-terminus, 7 TM domains, and C-terminal, cytoplasmic domain.
GABABRs are expressed in all major areas of the brain.
Recently GABABR2 (or GBR2) has been cloned from rat (940 aa) and human (941 aa).
GBR2 forms heterodimer with GBR1 through interaction at the intracellular C-terminal regions.
Heterodimerization appears to be important in transporting GBR1 to the membrane and expression of functional receptors.
GBR2 also colocalized with GBR1.
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blotting(1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL.
ELISAControl peptide can be used to coat ELISA plates at 1ug/ml and detected with antibodies (1:10-50K for neat serum and 0.5-1ug/ml for affinity pure).
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Purified using control peptide-Sepharose
Form：Supplied as a liquid, neat serum, 40% glycerol.
Specificity：The 18 AA rat control peptide is 100% conserved in human GBR1a (961 aa) and 94% in alternatively spliced GBR1c (899 aa). No significant homology is detected with GBR1b or GBR2 or other GABA receptors. The control peptide (mol wt. 1.78kD), because of its small size is not recommended for Western. It should be used in antibody blocking studies to confirm specificity of antibodies
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Rt
Immunogen：An 18 AA Peptide sequence near the N-terminus of rat GBR1a (1). The peptide was coupled to KLH or chickens
