GABA (g-amino butyric acid) is the most abundant neurotransmitter in mammalian brain.
GABA exerts its effects through ionotropic ligand-gated GABAA, GABAC and GABAB receptors (GABABRs).
A family of GABA-A receptors subtypes exists, which are generated by alternative splicing of alpha 1-6, beta 1-4, gamma 1-4, delta, epsilon, pie, theta, and rho1-3 to form a heteromeric (pentameric) protein complexes.
Various GABA-A subunits show distinct patterns of temporal and spatial expression that may imply its tissue specific physiological role (1).
GABA A (GAA) receptor proteins (450-627 aa) are characterized by the presence of a large cytoplasmic domain that may be involved in anchoring the receptor to the cytoskeleton.
Most recently, a new cellular protein, termed GABA A receptor associated protein (GABARAP), has bee shown to interact with gamma 2 subunit of GAA.
GABARAP has similarity with light chain-3 microtubule associated protein 1A and 1B.
N-terminus of GABARAP also possess a tubulin-binding motif.
Therefore, a possible interaction of GAA, GABARAP, and tubulin suggest for the targeting and clustering of GAA.
GABARAP is found in brain and most other tissues.
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blotting(1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL.
ELISAControl peptide can be used to coat ELISA plates at 1ug/ml and detected with antibodies (1:10-50K for neat serum and 0.5-1ug/ml for affinity pure).
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Purified using control peptide-Sepharose
Form：Supplied as a liquid, neat serum, 40% glycerol.
Specificity：The 19 AA human control peptide is 100% conserved in mouse and rat (2). The control peptide, because of its small size (<3kD) is not recommended for Western. It should be used in ELISA or antibody blocking studies to confirm specificity of antibodies
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A 19 AA Peptide sequence near the N-terminus of human GABARAP (2). The peptide was coupled to KLH
