GABA (g-amino butyric acid) is the most abundant neurotransmitter in mammalian brain.
GABA exerts its effects through ionotropic ligand-gated GABA-A, GABA-C and GABA-B receptors (GABAbetaRs).
A family of GABA-A receptors subtypes exists.
These receptors are generated by alternative splicing of alpha 1-6, beta 1-4, gamma 1-4, delta, epsilon, pie, theta and rho1-3 to form a heteromeric (pentameric) protein complexes.
Various GABA-A subunits show distinct patterns of temporal and spatial expression that may imply its tissue specific physiological role (1).
GABA A (GAA) receptor proteins (450-627aa) are characterized by the presence of a cleavable signal peptide, a large extracellular N-terminus, 3 TM (transmembrane) domains, a large cytoplasmic domain followed by TM4 and C-terminal extracellular domain.
The regions between TM3-4 and the large cytoplasmic loop are least conserved among various GAA subunits.
This variability may confer subunit specific functionality.
GAA genes are distributed as clusters throughout the human genome (chromosomes 4, 5, 15 and X; delta subunit on chromosome 1).
Human GAA1 (chromosome 5q34-q35) protein is 456aa (rat/mouse: 455aa).
Defects in GABRA1 are a cause of Juvenile Myoclonic Epilepsy (JME), a common epileptic syndrome characterized by febrile seizures, onset in adolescence (rather than in childhood) and myoclonic jerks.
GAA in the brain are the targets of many clinically important drugs.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 using ECL technique
ELISA: 1:10,000-1:50,000.
Coat plates at 1ug/ml with control peptide
Optimal dilutions to be determined by the researcher.
Control Peptide: G1015-06H
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：serum
Form：Supplied as a liquid, neat serum, 0.09% sodium azide, 40% glycerol.
Specificity：Recognizes human GAA1. No significant homology is detected with other GABA A receptor subtypes or other receptors. Species Crossreactivity: Mouse, rat, bovine and chicken.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Bo Ch Hu Mo Rt
Immunogen：Synthetic peptide 15aa sequence near the cytoplasmic, C-terminus of human GAA1 (KLH coupled).
