Elemental iron is required for a variety of normal cellular functions.
It is vital for proper growth and development.
Ferritin is the major protein involved in iron sequestration and detoxification.
Ferritin is found in all living species.
Mammalian liver and spleen ferritin (~450kD) consists of 24 subunits of 2 species.
The heavy subunit (~21kD; FTH) and the light subunit (~19kD; FTL).
The 2 types of apoferritin subunits were designated H and L for heart and liver, respectively.
Ferritin molecules from plants and bacteria contain only H-type chains.
'H-type' is associated with the presence of centers catalyzing the oxidation of two Fe(II) atoms.
FTL subunit (rich in human liver and spleen) is coded by a gene in segment 19q13.3.
FTH subunit (rich in human heart) is located on chromosome 11.
Ferritin is capable of storing up to 4500 atoms of ferric iron.
The H-to-L ratio within ferritin varies in a tissue-specific manner.
This ratio is also influenced by pathophysiological conditions, including inflammation and malignancy.
Hyperferritinemia-cataract syndrome has a mutation in the iron response element (IRE) in the 5-prime noncoding region of the FTL gene.
Synthesis of both ferritin subunits is controlled by a common cytosolic protein, iron regulatory proteins (IRPs), which binds to the iron-responsive element (IRE) in the 5'-UTR of the H- and L-ferritin mRNAs.
H-chains are important for Fe(II) oxidation.
L-chains assist in core formation.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1000-1:5000 antibody using ECL
ELISA: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Positive Controls:F4015-60: Ferritin L chain, Human, Control Peptide (FTL) F4015-75: Ferritin L-chain, Human, Western Blot Positive Control (FTL)
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.5, BSA.
Specificity：Recognizes Human FTL Light chain. No sequence homology with humna ferritin heavy chain.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide (15aa) near the NT of human FTL conjugated with KLH coupled. Species Squence Homology: Human FTH and FTL: 53%; porcine, rabbit ferritin l-chain: 100%; rat l-chain: 93%.
