Fatty acids are important for general cellular metabolism.
A number of proteins have been implicated in the transport and storage of fatty acid.
FABPs (fatty acid binding proteins) are a group of cytoplasmic, small mol wt (14-15kD).
FABP’s have a widespread tissue distribution.
FABPs are quite abundant (3-5% of total cellular protein).
At least 7 FABPs, FABP1-7, have been cloned and characterized from various tissues.
FABPs can bind long-chain fatty acid, fatty-acid acyl-CoA and acyl-L-carnitine.
Several different isoforms of FABP have been identified.
These isoforms are generally referred to by tissue type (liver, heart, intestine, adipocyte, kidney, brain, etc.
For example, protein designated as H-FABP indicates that it is heart type).
Expression of these isoforms is not exclusive.
More than one isoform can be found in a given cell or tissue.
Three main types of FABPs that were initially discovered in the heart (FABP-H), liver (FABP-L), and intestine (FABP-I) are not exclusive to these tissues and show considerable differences at the amino acid level (~30% identity).
Other FABPs detected in adipocyte, kidney, and brain show a high degree of sequence homology among each other and with other FABPs.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000
ELISA: 1:1,000-1:10,000.
Coat with 5-10ug F0019-77E
Optimal dilutions to be determined by the researcher.
Control Peptides:F0019-77E: Fatty Acid Binding Protein, Heart, Adipocyte, Human (FABP), Control PeptideF0019-77M: Fatty Acid Binding Protein, Human, Heart, Adipocyte, Human (FABP) Western Blot Control
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 0.09% sodium azide, 40% glycerol.
Specificity：Recognizes Fatty Acid Binding Protein (FABP), Heart, Adipocyte. Isoform Sequence Homology: Human adipocyte lipid binding protein (hALBP, 132aa), mouse/rat H-FABPs: 100%; human muscle FABP, porcine and bovine H-FABPs: 91%. No significant homology is seen with other isoforms of FABPs. Species Sequence Homology: The sequence is also quite conserved in species as diverse as fish and C. elegans FABP.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：12aa synthetic peptide corresponding to the C-terminus of human FABP (KLH).
