Mouse/rat ERb1 gene encodes a protein of 485aa with a calculated size of approx.
54kD (1).
Compared to ERa, ERb1 is highly conserved in the DNA-binding domain (> 90% homology) and the C-terminal ligand binding domain (55% homology).
The A/B domain, the hinge region and the F-domain are not conserved.
Rat ERb1 is primarily expressed in prostate, ovary, lung, bladder, brain, uterus and testis.
Most recently functional variants of ERb1, termed ERb2, have been cloned.
ERb2 has an additional 18aa as a results of in-frame mutation within the ligand-binding domain.
ERb2 is expressed in ovary, prostate, pituitary, brain and muscle.
Variants of both ERb1 and ERb2 (ERb1d3, ERb2d3) were detected that show deletion of 39aa in the DNA-binding domain.
Both ERb1 and ERb2 specifically bind to ER response element.
ERa, b1 and ERb2 have been found to heterodimerize with each other.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 1:100,000.
Use 50-100ng control peptide/well
Western Blot: 1:1000-1:5000.
Detects a band at ~54kD
Optimal dilutions to be determined by the researcher.
Control Peptide:E3565-17N: Estrogen Receptor b1, b2, Human (ERb1, 2) Control Peptide
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity：Recognizes human Estrogen Receptor b1, b2. It is not conclusive that all of the conserved ERb isoforms are visible and distinguishable by E3565-17L because of the very small size differences in the conserved isoforms.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：A 20 amino acid peptide located at the N-terminal of the human ERb conjugated to KLH. Species Sequence Homology: rat (84%). Sequence is also conserved in rat ERb2 and variants of ERb1/ERb2 (ERb1d3 & ERb2d3).
