Mouse/rat ERb1 gene encodes a protein of 485aa with a calculated size of approx.
54kD (1).
Rat ERb1 is highly conserved in the DNA-binding domain (>90% homology) and the C-terminal ligand binding domain (55% homology).
The A/B domain, the hinge region and the F-domain are not conserved.
Rat ERb1 is primarily expressed in prostate, ovary, lung, bladder, brain, uterus, and testis.
Most recently functional variants of ERb1, termed ERb2, have been cloned.
ERbb has an additional 18 aa as a results of in-frame mutation within the ligand-binding domain.
ERb1 is expressed in ovary, prostate, pituitary, brain, and muscle.
Variants of both ERb1 and ERb2 (ERb1d3, ERb2d3) were detected that show deletion of 39 aa in the DNA-binding domain.
Both ERb1 and ERb2 specifically bind to ER response element.
ERa, b1 and ERb2 have been found to heterodimerize with each other.
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
The predicted size of the ERb receptor is approx.
54-kD (1).
ELISA: ~1:100,000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, neat serum, 40% glycerol.
Specificity：The 16-aa mouse ERb11 immunogenic peptide shows 100% homology with the rat ERb1/ERb1d3 and 87% (14/16 AA) with human ERb1 and its variant ERb3. The ERB11-P sequence is also found in rat ERb2 and its variant ERb2d3 (3). It is not clear, at present, if all of these ERb isoforms can be seen and distinguished by this antibody because of very small size differences in various ERb isoforms. Actual crossreactivity of antibodies in all species is not established.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo Rt
Immunogen：A 16 AA peptide, near the C-terminal, steroid binding domain of the mouse ERb domain was synthesized, coupled to KLH.
