Tissue acidosis (decrease in pH below the physiological level) that occurs in ischemia, tissue damage or inflammation is accompanied by pain.
At the molecular level, H+-gated cation channels are activated by low pH in nociceptive neurons.
H+-gated cation channels, members of the NaC/DEG superfamily of Na channels that include amiloride-sensitive epithelial Na+ channel proteins (alpha, beta, and gamma, and delta-ENaC subunits), are expressed in epithelia of the vertebrate kidney, colon, lucolon and other tissues.
ENaC-b from various species are ~80% identical, and only ~30% similarity with the ENaC-alpha ENaC-delta (human 638 aa) is expressed mainly in brain, pancreas, testis, and ovary.
It is 27-30% homologous with the other ENaCs.
It can associate with b, and g ENaC to form a functional channel.
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000ELISA: 1:10,000-50,000, control peptide can be used to coat plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 0.05% sodium azide.
Specificity：Recognizes rat ENAC gamma. Species Sequence Homology: mouse (100%), human (92%), rabbit (80%). No significant homology is detected with ENAC alpha, beta, delta subunits.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo Rt
Immunogen：A 14 AA Peptide sequence near the N-terminus of rat ENAC g (1)
