Tissue acidosis (decrease in pH below the physiological level) that occurs in ischemia, tissue damage or inflammation is accompanied by pain.
At the molecular level, H+-gated cation channels are activated by low pH in nociceptive neurons.
H+-gated cation channels, members of the NaC/DEG superfamily of Na channels that include amiloride-sensitive epithelial Na+ channel proteins (alpha, beta, gamma and delta-ENAC subunits), are expressed in epithelia of the vertebrate kidney, colon, lung, tongue and brain.
The ENAC subunits may form heterotrimeric active Na channel.
ENACs are involved in Na and water readsorption, and salty taste transduction) of vertebrate colon, lung, kidney and tongue.
The superfamily of DEG/NAC proteins are characterized by intracellular N and C-termini, two TM domains, and a large extracellular loop.
ENAC-alpha subunits have been cloned from various tissues and from several species (human 669aa, rat 698aa, mouse 699aa; ~80% inter-species homology).
It controls the reabsorption of Na in kidney, colon, lung and sweat glands.
ENAC-alpha has also been implicated in taste perception.
Defects in ENAC-alpha are one of the cause of pseudohypoaldosteronism type I, a rare salt wasting disease characterized by dehydration, hyponatacemia, hyperkalemia and acidosis.
Inactivation of ENAC-alpha in transgenic mice causes an early death due to defective lung liquid clearance.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Control peptide can be used to coat ELISA plates at 1ug/ml and detected with antibodies.
Optimal dilutions to be determined by the researcher.
Control Peptide: E3414-61F.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Neat serum.
Form：Supplied as a liquid with 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes rat Epithelial Sodium Channel, alpha (ENAC Alpha). Species sequence homology: mouse-90%,; bovine-76%; human-75%; rabbit-70% ENACa. No significant homology is detected with ENAC beta, gamma or delta subunits.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：A 20aa peptide sequence near the N-terminus of rat ENACa.
