Prostanoids are the cyclooxygenase metabolites derived from C-20 unsaturated fatty acids (arachidonic acid) and include prostaglandin (PG) D2, PGE2, PGF2 alpha, PGI2, and thrombaxane (Tx) A2.
The fatty acids precursors are released from the membrane phosphoplipids in response to various physiological and pathological stimuli by the action of phospholipase A2 and are converted to various prostanoids by the sequential actions of cyclooxygenases and the respective synthases.
Prostaglandin PGE2 is one of the major prostaglandin produced during inflammation.
A variety of PGE2-mediated effects on vascular smooth muscle tonus, glomerular cell function, renin release, and renal salt and water transport have been described.
PGs also influence neuronal activity by modulating neurotransmitter release, sensitizing secretory fibers to noxious stimuli, or inducing fever and sleep.
The actions of PGE2 are mediated by rhodopsin-type; G-protein coupled membrane receptors, termed E-prostanoid (EP) receptors or PTGERs.
There are four subtypes of PGE receptors designated as EP1, EP2, EP3, and EP4 that are encoded by different genes and expressed differently in each tissue.
The intracellular signaling also differs among the receptor subtypes.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:ELISA: 0.5-1ug/ml.
Control peptide can be used to coat plate at 1ug/mlWestern Blot: 1-10ug/ml (ECL).
EP4 has been shown to be 47 and 78kD EP4 bands in the kidney
Optimal dilutions to be determined by the researcher.
Control Peptide:E3364-48: EP4 Receptor, Human, Control Peptide
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA.
Specificity：Recognizes human EP4 Receptor. No significant homology with other EP (1, 2 or 3) receptors.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：A synthetic peptide corresponding to 18aa near the C-terminal, cytoplasmic domain of human EP4, conjugated to KLH. Species Sequence Homology: rabbit, monkey and bovine (100%); rat, canine and mouse (94%)
