An intracellular protein termed ERAB (262aa, Endoplasmic reticulum associated Ab binding protein on chromosome X) has been cloned and linked with Ab neurotoxicity.
ERAB has structural homology with short-chain alcohol dehydrogenases (hydroxysteroid dehydrogenases and acetyl-CoA reductases).
Interestingly, ERAB localizes to endoplasmic reticulum despite the absence of a classical transmembrane sequence.
However, in the presence of Ab, ERAB-Ab complex translates to the inner plasma membrane.
Alzheimer patients have strong ERAB activity.
Normal brains have virtually no ERAB.
In addition, Ab neurotoxicity can be reduced by blocking ERAB and increased by its overexpression.
Applications:Suitable for use in Western Blot and ELISA.
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide: E2291-54A
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes human ERAB. Species Sequence Homology: Human probable short chain type alcohol-dehydrogenase/reductase and 3-hydroxyacyl-CoA dehydrogenase: 100%, Mouse ERAB: 100% (14/14aa), Rat ERAB: 93% (13/14aa).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to a 14aa sequence from the middle portion of human ERAB.
