An intracellular protein termed ERAB (262aa, Endoplasmic reticulum associated Ab binding protein on chromosome X) has been cloned and linked with Ab neurotoxicity.
ERAB has structural homology with short-chain alcohol dehydrogenases (hydroxysteroid dehydrogenases and acetyl-CoA reductases).
Interestingly, ERAB localizes to endoplasmic reticulum despite the absence of a classical transmembrane sequence.
However, in the presence of Ab, ERAB-Ab complex translates to the inner plasma membrane.
Alzheimer patients have strong ERAB activity.
Normal brains have virtually no ERAB.
In addition, Ab neurotoxicity can be reduced by blocking ERAB.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000.
Detects ~27kD protein in the brainELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Optimal dilutions to be determined by the researcher.
Recommended Control Peptide:E2291-54A: Endoplasmic Reticulum Associated Amyloid Beta Binding Protein, Human, Control Peptide
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 0.05% sodium azide.
Specificity：Recognizes human ERAB. Species Sequence Homology: Human probable short chain type alcohol-dehydrogenase/reductase and 3-hydroxyacyl-CoA dehydrogenase: 100%, mouse ERAB: 100% (14/14aa), rat ERAB: 93% (13/14aa).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to a 14aa sequence from the middle portion of human ERAB.
