The inhibitors of apoptosis proteins (IAPs) are a widely expressed gene family of apoptotic inhibitors.
Recently, a new human gene encoding a structurally and unique IAP designated Survivin has been identified.
Survivin (human 142 aa, ~16.5kD) is highly expressed in less-differentiated embryonic cells or rapidly dividing tumor cell but not in fully differentiated adult tissues.
Interestingly, Survivin coding strand has significant sequence homology with Effector cell Protease Receptor-1 (EPR-1) suggesting a potential for functional interaction between these two proteins
Factor Xa plays a critical role in the coagulation process by catalyzing the activation of prothrombin to thrombin.
It acts on leukocytes, endothelium and smooth muscle cells triggering complex pathways of intracellular signaling.
Factor Xa binds to EPR-1.
The full length EPR-1 predicts a protein of 337 aa (~65kD de-glycosylated form).
EPR-1 is expressed in vascular endothelial cells and smooth muscle cells.
Survivin and EPR-1 are encoded by structurally and topographically distinct messages from potentially originating from gene cluster at chromosome 17q25.
Overexpression of EPR-1 increased apoptosis and inhibited growth of transformed cells.
The importance of the Survivin-EPR-1 interaction remains to be elucidated.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 EPR-1 is ~62kD protein in detergent solubilized extracts of Jurkat and monocytic THP-1 cells
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Optimal dilutions to be determined by the researcher.
Control Peptide:E2212-53 Effector Cell Protease Receptor-1, Human Control Peptide
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS,, 0.05% azide, before the addition of glycerol to 40%
Specificity：Recognizes human Effector Cell Protease Receptor-1. Sequence homology: No homology with Survivin.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic 20-aa peptide within the C terminus of human EPR (1).
