Iron is an essential element for a variety physiological process.
A variety of proteins are involved in the transport of iron.
Most dietary iron exists in the form of ferric iron complexes which must be reduced to yield ferrous ion before it can be taken up by protein transporters such as DCT1 (divalent cation transporter)/NRAMP2/DMT1.
These transporters are especially active in small intestine where most dietary iron absorption is conducted.
Ferrous Fe (II) is very unstable at physiological pH and quickly oxidized to ferric Fe (III).
Therefore, highly specialized transmembrane electron transport system, maintained by ferric reductases, is required for the availability of intracellular ferrous ion.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Control peptide can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Recommended Control Peptide:D9830: Duodenal Cytochrome b, Mouse, Control Peptide (Dcytb)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity：Recognizes mouse Duodenal Cytochrome b (Dcytb). No significant sequene homology exists with b558 or other reductases.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide consisting of 20aa sequence located within the cytoplasmic C-terminus of mouse DCYTB (KLH). Species sequence homology: human: 70%.
