84-1042-80　Dopamine Transporter (DAT) 100ul　D9020-18

特徴

• Dopamine is an endogenous catecholamine that influences many cellular activities.
• A family of at least 5 Dopamine Receptors (DR) genes, D1-D5, have been identified based on the aa identity, pharmacological specificity and effector responses.
• DR have been classified into either the D1-like (D1, D1B and D5) or D2-like (D2, D3 and D4).
• The two isoforms of D2R, D2 long (D2L) and short (D2S), are encoded by splice variants of a single gene and differ only by the presence of an additional 29aa in the intracellular domain 3 of the D2 long form.
• All members of this family have similar structure and contain 7 putative transmembrane domains.
• A given cell may express more than one DR.
• Specific radioligands do not exist that can differentiate between these DR.
• Therefore, specific antibodies are needed to distinguish, localize, and document changes in DR levels in cells and tissues under various normal and pathological conditions.
• Dopamine transporter (DAT) is plasma membrane transporter for the termination of dopaminergic neurotransmission by its high affinity Na+ and Cl- reuptake of dopamine into presynaptic terminals.
• This protein is the target of psychomotor stimulants such as amphetamines or cocaine.
• DAT (human 620aa; rat/mouse 619aa glycoprotein) is predicted to contain 12 transmembrane domains with N- and C-termini in the cytoplasm.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilutions:ELISA: 1:10,000-1:50,000 Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.