84-1042-73　Dopamine Receptor, D3, D3R 100ug　D9017-25

特徴

• Lyophilized products should be reconstituted in 100 ul water and gently mixed for 15 min at room temp.
• All peptide/antibody received in solution or reconstituted from lyophilized vials should be stored frozen at -20°C or below in suitable aliquots.
• It is not recommended to store diluted solutions.
• Avoid repeated freeze and thaw.
• Applications:Suitable for use in ELISA, Western Blotting, Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL.
• Approx.
• 100-400ug membrane protein/lane should be loaded to visualize the receptor band
• ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure.
• Control peptide can be used to coat ELISA plates at 1ug/ml
• Immuoprecipitation: Not tested.
• We suggest 5-10 ul neat serum or 1-10ug affinity pure per 100-400ug of protein samples.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.