Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL.
Approx.
100-400ug membrane protein/lane should be loaded to visualize the receptor band
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure.
Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, neat serum, 40% glycerol.
Specificity：The 19 AA rat D3R peptide showed no significant sequence homology with other dopamine receptors (D1, D2, D4 or D5). It has sequence homology of 100% with mouse (19/19 AA) and 63% with human/monkey (12/19 AA) D3R receptors (1-2). Antibody crossreactivity with D3R from various species is not known
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：A 19 AA Peptide (Control peptide) sequence from the rat D3 receptor within the 3rd cytoplasmic domain. The peptide was coupled to KLH
