Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml (ECL) The predicted size of D2 form is approx.
50kD.
However, additional bands at approx.
100kD, 68kD, and 50kD, and others are typically seen in various tissues (2-3).
Approx.
100-400ug membrane protein/lane should be loaded to visualize various bands
ELISA: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Control Peptide: D9016-45
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in TBS, pH 7.5 and 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes human D2R. No significant sequence homology is seen with other dopamine receptors (D1 and D3-D5).Species sequence homology: human, bovine, canine-100%; xenopus-50% D2 receptors. Species Crossreactivity: human, bovine, canine.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Bo Ca Hu
Immunogen：A 28 AA synthetic peptide sequence from the human D2 R, within the cytoplasmic loop # 3, coupled to KLH (2). The 28 AA sequence is shared by both the long and short form.
