Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport.
A family of at least 5 Dopamine Receptors (DR) genes, D1-D5, have been identified based upon the amino acid identity, pharmacological specificity and effector responses.
DR have been classified into either the D1-like (D1, D1B, and D5) or D2-like (D2, D3, and D4).
Dopamine D2 receptor is known to exist in the short (D2S) and the long (D2L)forms that are encoded by splice variants of a single gene and differ only by the presence of an additional 29 amino acid in the intracellular, cytoplasmic loop 3.
D2L receptor is a 446 aa, G- protein coupled, and transmembrane receptor protein.
D2R may play a role in the coupling of the receptor to G-proteins.
All members of this family have similar structure and contain 7 putative transmembrane domains.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilutions:ELISA: 0.5-1 ug/ml.
Control peptide can be used to coat the plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Control Peptide:D9016-42: Dopamine Receptor, Rat, Control Peptide, D2R
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder, 01% BSA. Reconstitute with 100ul sterile PBS.
Specificity：Recognizes rat Dopamine Receptor, D2R. Species Crossreactivity: human (CNS and peripheral), lobster, squid and crawfish.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide coresponding to 11aa peptide near the N-terminal, extracellular domain 1 of rat D2R, conjugated to KLH. Species Sequence Homology: mouse; 100%, human and bovine; 90%, canine, 72%.
