Dopamine D2 receptor is known to exist in the short (D2S) and the long (D2L)forms that are encoded by splice variants of a single gene and differ only by the presence of an additional 29 amino acid in the intracellular, cytoplasmic loop 3 (1).
D2L receptor is a 446 aa, G-protein coupled, and transmembrane receptor protein (2).
Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport.
A family of at least 5 Dopamine Receptors (DR) genes, D1-D5, have been identified based upon the amino acid identity, pharmacological specificity and effector responses.
DR have been classified into either the D1-like (D1, D1B, and D5) or D2-like (D2, D3, and D4).
The two isoforms of D2R, D2 long (D2L) and short D2S), are encoded by splice variants of a single gene and differ only by the presence of an additional 29aa in the intracellular domain 3 of the D2 long form.
It may play a role in the coupling of the receptor to G-proteins.
All members of this family have similar structure and contain 7 putative transmembrane domains.
A given cell or tissue may express more than one DR.
Specific radioligands do not exist that can differentiate between these DR.
Therefore, specific antibodies are needed to distinguish, localize, and document changes in DR levels in cells and tissues under various normal and pathological conditions.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml for affinity pure antibody using ECL.
The predicted size of D2L form is approx.
50kD.
However, additional bands at approx.
50kD, 100kD, and 60kD, and others are typically seen in various tissues (2-3).
Approx.
100-400ug membrane protein/lane should be loaded to visualize various bands
ELISA: 0.5-1ug/ml for affinity pure.
Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified over the peptide-Sepharose column
Form：Supplied as a liquid in PBS, pH 7.4 and 0.1% BSA, 0.09% sodium azide, 40% glycerol.
Specificity：The 27 AA rat peptide was found only in the D2 long form. No significant sequence homology is seen with other dopamine receptors (D1, D2 short and D3-D5). The 27 AA rat D2RL peptide shows sequence homology of 96% with human/bovine (26/27 AA), 62% with xenopus (17/27 AA) D2L receptors. Antibodies generated to the rat sequence have been shown to recognize the canine D2R (2). Antibody crossreactivity with D2L receptors from various species is not known
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：A 27 AA Peptide (Control peptide) sequence from the rat D2 long form, within the cytoplasmic loop # 3, (2). The 27 AA sequence is unique to the long form and it is not found in the D2 short or other receptor isoform. The peptide was coupled to KLH
