Several endogenous factors have been linked to rhythmicity or circadian behavior of living organisms.
In Drosophila, the genes period (dPer) and timeless (tim), and in Neurospora frequency (frq), have been proposed to be responsible for their circadian rhythm.
Recently human and mouse genes encoding a basichelix-loop-helix (bHLH) and Per-ARNT-Sim (PAS)-domain with significant similarity to the Drosophila Period have been reported.
The cDNA sequences of hPER and mPer1 (also named RIGUI) are predicted to encode for proteins of length 1290 and 1291 amino acids respectively.
Homologues of mPer1 designated Per 2 (1257 aa) and Per3 (1113 aa) have also been cloned.
Both Per1 and Per2 levels show circadian rhythm in the SCN and eyes.
It has been suggested that mPer regulates neuronal activity in the SCN.
Using genetic approach, a single mutation (A to T in the Clock gene affects circadian rhythmicity in mice.
Clock has been mapped to chromosome 5.
Mouse Clock encodes a transcription factor, a single polypeptide chain of 855 aa (predicted calculated mol wt ~97kD; pI 6.52; hClock, 846 aa).
Clock is abundantly expressed in brain (SCN, pyrifom cortex, hippocampus) as well as in other tissues (eye, total brain, tested, ovaries, liver, heart, lung, and kidney).
Although, Clock is constitutively expressed (not rhythmic) in the SCN, it may still be an important component of circadian machinery.
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blotting, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL(2).
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Immunohistochemistry & Immunofluorescence: We recommend the use of affinity purified antibody at 2-20ug/ml.
Frozen, free floating sections (2).
Optimal dilutions to be determined by the researcher.
Control peptide, because of its low MW (<3kD), is not suitable for Western.
It is recommed for ELISA, dot blot, or for antibody blocking experiments (use 5-10 ug of control peptide per 1 ug of affinity purified IgG or 1 ul of antiserum) to demonstrate antibody specificity.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder.
Specificity：Human peptide is 55% conserved zebra fish, 50% in Japanese quail and mouse Per3. We recommend the use of antibody for mouse Per3. Antibody crossreactivity in various species is not established.
Isotype：IgG
Calc Applications Abbrev：DB E WB
Calc Crossreactivity：Hu
Immunogen：A 20-aa peptide sequence within C-terminus of human Per3 (1) was synthesized coupled to KLH.
