Deoxyribonuclease II (Dnase II, EC.3.1.22.1) is one of the two Dnase present in mammalian tissues and biological fluids.
Dnase II hydrolyzes DNA to 3’-phosphoryl oligonucleotides under acidic conditions.
Dnase II is also known as “acid Dnase”.
It has preference of for dsDNA.
Dnase II has been implicated in internucleosomal cleavage of chromatin to generate DNA fragments or ladder, a hallmark of apoptosis.
Dnase II (mouse 353aa; human 360aa, chromosome 19p13.2-q13.4) is a lysosomal enzyme present in most tissues.
Dnase Ii is synthesized as a pro-enzyme (signal peptide aa1-16, propeptide region of 91-aa, and 253-aa mature protein (aa110-353) and processed to produce active enzyme containing 2- chains of ~32 and 12kD.
Dnase II has no significant sequence homology with Dnase I.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000-1:50,000 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, pH 7.4, 0.1% BSA
Specificity：Recognizes mouse Deoxyribonuclease II (Dnase II). The peptide sequence is not well conserved in human Dnase II. No significant sequence homology with other lysosomal enzymes.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：A synthetic peptide corresponding to 17aa near the C-terminus of mouse Dnase II, conjugated to KLH
