84-1042-45　［取扱停止］Cryptochrome 2 (CRY2) 100ul　C7942-50

特徴

• Circadian rhythm is one of the most fascinating and complex biological phenomenon.
• The circadian clock controls biological activities on daily light-dark cycles in species from cyanobacteria to humans.
• The circadian clock has three major components: A photoactive pigment (chromophore) for sensing light and transmitting light signals, the circadian clock that oscillates every ~24-hrs, and the genes controlled by the circadian clock to bring about the physiological and behavioral changes.
• Several genes (Drosophila Clock Per, Tim; mammalian Per1, Per2, Per3, MOP3 and MOP4, and BMAL1) have been linked to rhythmicity or circadian behavior of living organisms.
• However, very little is known about the mammalian photosensory molecules
• Most recently mammalian homolog of the plant blue-light photoreceptors termed cryptochromes have been identified.
• Mouse CRY1 and CRY2, are 606 aa and 569 aa protein, respectively.
• Cry1 and Cry are specifically expressed in ganglion cell and inner nuclear layers of the mouse retina.
• CRY1 is expressed at high level in the SCN and oscillates in a circadian manner.
• Like other genes implicated in circadian mechanism, CRY1 and CRY2 are also expressed in most animal tissues.
• Therefore, mammalian cryptochromes appears to play an important role in entrainment of the circadian clock.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000
• ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:For long-term storage, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.