Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using Chemiluminescence technique.
The antibodies detected proteins of ~63-65kD in rat lens.
The same set of bands were detected by anti-MP70
ELISA: 1:10K-100K using 50-100ng of the control peptide per well
Optimal dilutions to be determined by the researcher.
Control Peptide:C7859-05B Connexin 50, Mouse Control Peptide
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
Specificity：Reecognizes mouse Connexin 50 (CX50, Gap Junction Alpha 8 Protein, CXA-8, Lens Fiber Protein MP70). Species sequence homology: rat 100%. No reactivity observed with other connexins.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo
Immunogen：A 13aa peptide sequence within the 2nd cytoplasmic domain (between 2nd and 3rd transmembrane domain) (KLH).
