Gap junctions are composed of transmembrane channels that link the cytoplasm of neighboring cells.
They differ from other membrane channels since they exist between two cells.
Gap junctions are relatively non-specific and allow passive diffusion of small molecules up to 1000 Dalton.
The junctions exist in almost all vertebrate and non-vertebrates cells.
It is believed that gap junction play an important for intercellular communications and affect growth and differentiation of cells.
Gap junctional channel is composed of a hemichannel (connexon) in the cell membrane of one cell joined in mirror symmetry with a connexon in the opposing cell.
Each connexon is an oligomer of six protein subunits that define the axial aqueous pore.
Molecular cloning studies have identified a family of at least 12 highly related Connexins that are designated according to MW, Cx26-50.
Hydropathy analyses of Cx sequences predicts 4 transmembrane ™, 2 extracellular (EC), and 3 cytoplasmic (CP) domains.
The EC, TM, and N-terminal CP domains are well conserved among family members, while Central and C-terminal domains are highly variable in both sequence and size.
The N and C-termini are predicted to be cytoplasmic.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000ELISA: 1:100,000 using 50-100ng control peptide/well (C7858-05E).
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, neat serum, 40% glycerol.
Specificity：The mouse Cx45 peptide sequence is 100% homologous in chicken, canine, human and mouse (AA 285-298) Cx45 (4). It has no significant homology with other Connexins
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：A 14 amino acid peptide sequence near the C-terminal, cytoplasmic domain of the mouse Cx45 (2) was used to generate anti-mouse Cx45. A cysteine has been added at the NH2 terminus for coupling to KLH. The peptide was coupled to KLH
