Rat Connexin 37 is A 333 aa gap junction protein with a predicted MW.
Of ~37kD.
It is prominently expressed in lung (see reviews in refs.
1).
A 19 amino acid peptide sequence within the C-terminal cytoplasmic domain of rat Cx37 (2) was used to generate anti-Mouse Cx37.
Peptide was coupled to KLH (carrier protein) Via the N-terminal Cysteine and antibodies generated in rabbits.
Applications:Suitable for use in ELISA, Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: We recommend testing of antiserum at 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using Chemiluminescence technique.
ELISA (1:10K-100K using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes rat Cx37. No significant homology is seen with other Connexins. Species Sequence Homology: mouse, 94%; human, 52%.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide (19aa) near the C-terminus of rat CX37 (KLH).
