Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL
ELISA: 1:100,000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Control Peptide: C7853-44
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.02% sodium merthiolate, 40% glycerol.
Specificity：Recognizes rat Cx30.3 (predicted MW: 31kD). Actual crossreactivity of antibodies in various species is not known.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：A 20 amino acid peptide sequence within the first cytoplasmic domain of rat Cx30.3. The peptide was coupled with KLH (carrier protein) using NH2-terminal Cysteine. The peptide sequence is identical in mouse Cx30.3 and has no significant homology with other connexins.
