Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:5000ELISA: 1:100,000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Control Peptide: C7853-44
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ul
Host:rabbit
Source Antibody:rat
Grade:Serum
Purity:Serum
Form:Supplied as a liquid, neat serum, with 40% glycerol
Specificity:Recognizes rat Cx30.3 (predicted MW: 31kD). Actual crossreactivity of antibodies in various species is not known.
Isotype:IgG
Calc Crossreactivity:Rt
Immunogen:A 20 amino acid peptide sequence within the first cytoplasmic domain of rat Cx30.3. The peptide was coupled with KLH (carrier protein) using NH2-terminal Cysteine. The peptide sequence is identical in mouse Cx30.3 and has no significant homology with other connexins.