Gap junctions are composed of transmembrane channels that link the cytoplasm of neighboring cells.
They differ from other membrane channels since they exist between two cells.
Gap junctions are relatively non-specific and allow passive diffusion of small molecules up to 1000D.
The junctions exist in almost all vertebrate and non-vertebrates cells.
It is believed that gap junction play an important for intercellular communications and affect growth and differentiation of cells.
Gap junctional channel is composed of a hemichannel (connexon) in the cell membrane of one cell joined in mirror symmetry with a connexon in the opposing cell.
Each connexon is an oligomer of six protein subunits that define the axial aqueous pore.
Molecular cloning studies have identified a family of at least 12 highly related Connexins that are designated according to MW, Cx26-50.
Hydropathy analyses of Cx sequences predicts 4 transmembrane (TM), 2 extracellular (EC), and 3 cytoplasmic (CP) domains.
The EC, TM, and N-terminal CP domains are well conserved among family members, while Central and C-terminal domains are highly variable in both sequence and size.
The N and C-termini are predicted to be cytoplasmic.
Applications:Suitable for use ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL Recognizes a band of ~25kD in mouse liver.
ELISA: 1:100,000 using 50-100ng CX26 control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes mouse connexin 26.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：A 19 amino acid peptide sequence near the C-terminal, 2nd cytoplasmic domain of the mouse Cx26 (KLH coupled). Species sequence homology: rat (100%), monkey (94%), human (89%), sheep, bovine and dog (8%), rat CX30 (63%). No significant hmology with other connexins.
