The cyclic nucleotides cAMP and cGMP are implicated in signal transduction events such as the visual transduction, relaxation of smooth muscles, intestinal secretion of water and salt, reabsorption of Na+ and water in the distal tubule of the nephrons.
cAMP/cGMP activate Ca2+-permeable ion channels called cyclic nucleotide-gated channels (CNG or CNC).
Activation of CNG leads to depolarization of the membrane voltage and to a concomitant increase of the cytosolic Ca2+.
CNG consists of two distinct subunits, designated a and b subunits.
Several CNG a-subunits (CNG alpha 1-3) & beta subunits (subunit 2 or CNGb1-2 or CNG4-5) and numerous isoforms.
a-subunit can form functional channel by themselves, whereas, b-subunits modulate the channel property of a-subunits.
CNG display intracellular N and C-termini, 6 transmembrane domains or segments (S1-S6).
The region between S5 and S6 contains the ion-conducting pore (P).
The cyclic nucleotide-binding region is found at the c-terminus.
Native functional CNG may exists as heteromultimer containing some combination of a, and b subunits.
CNG2 or OCNC1 (rat/mouse/rabbit 664aa, bovine 663aa) is primarily expressed in olfactory sensory neurons.
It is ~80% homologous with CNG1.
Unlike CNG1, CNG2 is activated both by cAMP and cGMP.
Another cGMP-gated channel called CNG3 (mouse 537aa, rat 611aa, human, 694aa, bovine 706aa, chicken 645aa) has been cloned from heart, kidney, testis, sperm, and taste buds.
Deletion of CNG3 gene in mice leads to degeneration of cone photoreceptors
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECLELISA: 0.5-1ug/ml.
Coat ELISA plates at 1ug/ml with Control Peptide.
Optimal dilutions to be determined by the researcher.
Control Peptide: C6000-07
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Specificity：Recognizes rat CNG2. No significant sequence homology is detected with other CNGs. Species crossreactivity: Mouse (100%); bovine and rabbit: (94%).
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo Rt
Immunogen：Synthetic peptide sequence (18aa) near N-terminus of rat/mouse CNG2 (KLH coupled).
