The cyclic nucleotides cAMP and cGMP are implicated in signal transduction events such as the visual transduction, relaxation of smooth muscles, intestinal secretion of water and salt, reabsorption of Na+ and water in the distal tubule of the nephrons.
cAMP/cGMP activate Ca2+ -permeable ion channels called cyclic nucleotide-gated channels (CNG or CNC).
Activation of CNG leads to depolarization of the membrane voltage and to a concomitant increase of the cytosolic Ca2+.
CNG consists of two distinct subunits, designated a and b subunits.
Several CNG a-subunits (CNG alpha 1-3) & beta subunits (subunit 2 or CNGb1-2 or CNG4-5) and numerous isoforms.
a-subunit can form functional channel by themselves.
b-subunits modulate the channel property of a-subunits.
CNG display intracellular N and C-termini, 6 transmembrane domains or segments (S1-S6).
The region between S5 and S6 contains the ion-conducting pore (P).
The cyclic nucleotide-binding region is found at the c-terminus.
Native functional CNG may exists as heteromultimer containing some combination of a, and b subunits.
CNG2 or OCNC1 (rat/mouse/rabbit 664 aa, bovine 663 aa) is primarily expressed in olfactory sensory neurons.
It is ~80% homologous with CNG1.
Unlike CNG1, CNG2 is activated both by cAMP and cGMP.
Another cGMP-gated channel called CNG3 (mouse 537aa, rat 61 aa, human, 694aa, bovine 706aa, chicken 64 aa) has been cloned from heart, kidney, testis, sperm, and taste buds.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Optimal dilutions to be determined by the researcher.
Recommended Control Peptide: C6000-07
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity：Recognizes rat CNG2. No significant sequence homology is detected with other CNGs.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide corresponding to 18aa sequence near the N-terminus of rat/mouse CNG2 (KLH). Species sequence homology: mouse (100%); bovine and rabbit: (94%).
