CLOCK, a basic helix-loop-helix (bHLH)/PAS protein, is an important factor in the molecular clock mechanism.
Mutations in this protein lead to abnormal circadian behavior and defective transcriptional activity.
CLOCK has been shown to interact strongly with other bHLH-PAS proteins such as MOP3, a BMAL1 isoform
Several endogenous factors have been linked to rhythmicity or circadian behavior of living organisms.
In Drosophila, the genes period (dPer) and timeless (tim), and in Neurospora frequency (frq), have been proposed to be responsible for their circadian rhythm.
Recently human and mouse genes encoding a basic helix-loop-helix (bHLH) and Per-ARNT-Sim (PAS)-domain with significant similarity to the Drosophila Period have been reported.
The cDNA sequences of hPER and mPer1 (also named RIGUI) are predicted to encode for proteins of length 1290 and 1291 amino acids respectively.
Homologues of mPer1 designated Per 2 (1257 aa) and Per3 (1113 aa) have also been cloned.
Both Per1 and Per2 levels show circadian rhythm in the SCN and eyes.
It has been suggested that mPer regulates neuronal activity in the SCN.
Using genetic approach, a single mutation (A to T in the Clock gene affects circadian rhythmicity in mice.
Clock has been mapped to chromosome 5.
Mouse Clock encodes a transcription factor, a single polypeptide chain of 855 aa (predicted calculated mol wt ~97kD; pI 6.52; hClock, 846 aa).
Clock is abundantly expressed in brain (SCN, pyrifom cortex, hippocampus) as well as in other tissues (eye, total brain, tested, ovaries, liver, heart, lung, and kidney).
Although, Clock is constitutively expressed (not rhythmic) in the SCN, it may still be an important component of circadian machinery
Basic-helix-loop-helix-PAS orphan protein, MOP3 (Members Of PAS Superfamily; also known as BMAL1/JAP3/PAS3; 626 aa) is a general dimerization partner for several PAS superfamily of transcription regulators.
MOP3 interacts with MOP4 (also known as NPAS2; 626 aa), Clock, HIF1a, and HIF2a.
MOP4 is a brain specific homolog of Clock.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：drosophila
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide
Specificity：Recognizes Drosophila Clock. Drosophila sequence has no significant sequence homology with mammalian Clock or Per proteins.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Dr
Immunogen：Synthetic peptide corresponding to 17aa within the C-terminus of Drosophila Clock conjugated to KLH.
