84-1041-76　［取扱停止］CLC7 (Clcn7, Chloride Channel) 100ul　C5837-58

特徴

• Chloride is a critical component of all living cells.
• Voltage-gated chloride channels regulate cellular traffic of chloride ion.
• The chloride channels (CIC or CLC) performs several functions including the regulation of cell volume, membrane potential stabilization, signal transduction, and transepithelial transport.
• Mutations in CIC genes have been linked with several human diseases including myotonias (Thomsen's disease), cystic fibrosis, Bartters syndrome type III, Dent's disease, and X-linked recessive nephrolithiasis.
• In mammals, CLC proteins form a superfamily of at least 9 different genes (CLC1-7 also known as CLCN1-7 and CLK1-2 or CLCKa and CLCKb).
• Additional forms of these proteins are obtained by alternative splicing.
• All CLC proteins (~700-1000 aa) are predicted to contain 10 (possibly 12) transmembrane domains.
• Except CLC-1 and CLC-K1/K2 that are specific for kidney, most other CLC are widely distributed in various tissues.
• Rat CLC-7 is 802 aa membrane protein (mouse 803 aa, human 805 aa) (1).
• CLC-7 has a calculated MW of approx.
• 90kD.
• CLC-7 is ~45% identical with CLC-6.
• CLC-7 is expressed in brain, tested, muscle and kidney (1).
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:10,000-50,000Western Blot: 1:1000-5000Optimal dilutions to be determined by the researcher.
• Control Peptide:C5837-56
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.