84-1041-75　［取扱停止］CLC7 (Clcn7, Chloride Channel) 100ug　C5837-52

特徴

• Chloride is a critical component of all living cells.
• Voltage-gated chloride channels regulate cellular traffic of chloride ion.
• The chloride channels (CIC or CLC) performs several functions including the regulation of cell volume, membrane potential stabilization, signal transduction and transepithelial transport.
• Mutations in CIC genes have been linked with several human diseases including myotonias (Thomsen's disease), cystic fibrosis, Bartters syndrome type III, Dent's disease and X-linked recessive nephrolithiasis.
• In mammals, CLC proteins form a superfamily of at least 9 different genes (CLC1-7 also known as CLCN1-7 and CLK1-2 or CLCKa and CLCKb).
• Additional forms of these proteins are obtained by alternative splicing.
• All CLC proteins (~700-1000aa) are predicted to contain 10 (possibly 12) transmembrane domains.
• CLC-1 and CLC-K1/K2 that are specific for kidney.
• Most other CLC are widely distributed in various tissues.
• Rat CLC-7 is 802aa membrane protein (mouse 803aa, human 805aa) (1).
• CLC-7 has a calculated MW of ~90kD.
• CLC-7 is ~45% identical with CLC-6.
• CLC-7 is expressed in brain, testes, muscle and kidney (1).
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-10ug/ml (ECL).
• CLC7 is ~80kD (2)
• ELISA: 0.5-1ug/ml.
• Control peptide can be used to coat ELISA plates at 1ug/ml
• Optimal dilutions to be determined by the researcher.
• Control Peptide:C5837-54: CLC7, Rat (Clcn7, Chloride Channel)
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.